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Isolation and characterization of microsatellites in Bambusa arundinacea and cross species amplification in other bamboos

Nayak, S., Rout, G.R.
Plant breeding 2005 v.124 no.6 pp. 599-602
Bambusa bambos, Dendrocalamus, Bambusa, Arundinaria, microsatellite repeats, genetic markers, polymerase chain reaction, genetic polymorphism, loci, alleles, DNA primers, nucleotide sequences, genetic variation
Isolation and characterization of microsatellites was analysed in Bambusa arundinacea and cross species amplification studied in other bamboos. Microsatellites, tandem repeats of short nucleotide (1-6 bp) sequences, are the DNA marker of choice because of their highly polymorphic, ubiquitous distribution within the genome, ease of genotyping through Polymerase chain reaction, selectively neutral, co-dominant and multiallelic nature. Six microsatellites, three polymorphic and three monomorphic have been characterized for the first time in a bamboo species, Bambusa arundinacea belonging to the family Poaceae. The numbers of alleles per locus ranged from 2 to 13. Cross species amplification was tested in 18 other bamboo species. Monomorphic simple sequence repeats (SSRs) were found to be cross amplified in most of the species tested and polymorphic ones in only three to four species. The utility of SSR loci in a genetic diversity study of B. arundinacea and other cross-amplified bamboo species has been discussed. This study will help in population genetic studies in bamboo species.