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Development of reliable PCR markers for the selection of the Vf gene conferring scab resistance in apple

Tartarini, S., Gianfranceschi, L., Sansavini, S., Gessler, C.
Plant breeding 1999 v.118 no.2 pp. 183-186
random amplified polymorphic DNA technique, Venturia inaequalis, Malus floribunda, fungal diseases of plants, genes, disease resistance, Malus domestica, linkage (genetics), genetic markers
Early selection of scab-resistant apple seedlings can be enhanced by the use of markers tightly linked to the Vf resistance gene. Two sequence characterized amplified regions (SCAR) markers have been obtained from previously described random amplified polymorphic DNA (RAPD) markers. AL07-SCAR is a codominant marker, while AM19-SCAR is dominant, as is the RAPD from which it was derived. A highly detailed map in the vicinity of the Vf gene was built through the cumulative analysis of about 600 seedlings from six different controlled crosses. The usefulness of these and other SCAR markers will be discussed in relation to combining the traditional phenotypic selection with MAS. The availability of two codominant, tightly linked markers flanking both sides of the resistance gene (AL07-SCAR and M18-CAPS) also makes it easy to identify the seedlings homozygous for the resistance gene.