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Molecular evidence that the whitefly-transmitted sweetpotato mild mottle virus belongs to a distinct genus of the Potyviridae
- Colinet, D., Kummert, J., Lepoivre, P.
- Archives of virology 1996 v.141 no.1 pp. 125-135
- Potyvirus, nucleotide sequences, polymerase chain reaction, amino acid sequences, Nicotiana, phylogeny, new genus, coat proteins, DNA-directed RNA polymerase, genomics, Nicotiana benthamiana, species differences
- Complementary DNA representing 2 108 nucleotides at the 3' end of the genomic RNA of the whitefly-transmitted sweetpotato mild mottle virus (SPMMV) was cloned after PCR. Sequence analysis revealed an open reading frame of 1 797 nucleotides which codes for a protein of 599 amino acids, followed by a 3' non-coding region of 311 nucleotides. Alignment of the deduced amino acid sequence with corresponding sequences of other members of the Potyviridae demonstrated that part of the presumptive RNA-dependent RNA polymerase and the coat protein coding regions of SPMMV are found at the 3' end of its genome, in that order. Alignment of the amino acid sequence of the core of SPMMV coat protein with those of selected members of the Potyviridae showed limited identity, thus demonstrating - with phylogenetic analysis- that SPMMV belongs to a distinct genus of the family Potyviridae.