Main content area

Interferon-gamma is expressed in both gut and spleen during Eimeria tenella infection

Rothwell, L., Muir, W., Kaiser, P.
Avian pathology 2000 v.29 no.4 pp. 333-342
chickens, Eimeria tenella, cecum, spleen, splenocytes, messenger RNA, immune response, coccidiosis, interferons
The effect of primary infection and subsequent challenge with Eimeria tenella on interferon-gamma (IFN-gamma) production in the spleen and caeca of Light Sussex chickens was assessed. The ability of splenocytes to proliferate and produce IFN-gamma in response to mitogen stimulation ex vivo was determined. Differences in the kinetics of IFN-gamma production suggested that the spleens of infected birds contain a subpopulation of T cells, primed to produce IFN-gamma, which migrate from the spleen in response to a secondary infection. IFN-gamma mRNA expression was detected by hybridization of an anti-sense chicken IFN-gamma riboprobe to splenic sections from infected birds and caecal sections from challenged birds. Hybridization was to T-cell areas in the spleen, and to cells in the lamina propria and intraepithelial compartments of the caecum. This is the first direct demonstration of IFN-gamma expression in chickens at the site of E. tenella infection, and also the first indication that IFN-gamma may be involved in the immune response to challenge.