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The effect of immobilization on recombinant protein production in plant cell culture
- Bodeutsch, T., James, E.A., Lee, J.M.
- Plant cell reports 2001 v.20 no.6 pp. 562-566
- Nicotiana tabacum, recombinant proteins, animal proteins, cultured cells, protein synthesis, encapsulation, alginates, colony-stimulating factors, volume, immobilized cells
- The effects of encapsulation on the production of recombinant human proteins by Nicotiana tabacum cells were investigated using alginate, carrageenan, and agar as immobilization matrices. Experiments showed that cell encapsulation in alginate increased the production of human granulocyte-macrophage colony-stimulating factor (GM-CSF) in tobacco cells by approximately 50%. Alginate also yielded the highest quality beads and the most reproducible growth results. The most likely cause for this increased protein production is the altered growth conditions within the alginate beads resulting in a prolonged exponential growth phase. To characterize these effects, we compared growth performance and protein production for various gel geometries, bead sizes, and volume fractions of beads.