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The regulation of circadian period by phototransduction pathways in Arabidopsis

Author:
Millar, A.J., Straume, M., Chory, J., Chua, N.H., Kay, S.A.
Source:
Science 1995 v.267 no.5201 pp. 1163-1166
ISSN:
0036-8075
Subject:
Arabidopsis thaliana, transgenic plants, gene expression, luciferase, reporter genes, recombinant DNA, luminescence, mutants, circadian rhythm, phytochrome, blue light, red light, loci, promoter regions, mutation
Abstract:
Transgenic Arabidopsis plants expressing a luciferase gene fused to a circadian-regulated promoter exhibited robust rhythms in bioluminescence. The cyclic luminescence has a 24.7-hour period in white light but 30- to 36-hour periods under constant darkness. Either red or blue light shortened the period of the wild type to 25 hours. A phytochrome-deficient mutation lengthened the period in continuous red light but had little effect in continuous blue light, whereas seedlings carrying mutations that activate light-dependent pathways in darkness maintained shorter periods in constant darkness. These results suggest that both phytochrome- and blue light-responsive photoreceptor pathways control the period of the circadian clock.
Agid:
1433944