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Methylmalonate-semialdehyde dehydrogenase is induced in auxin-stimulated and zinc-stimulated root formation in rice
- Oguchi, K., Tanaka, N., Komatsu, S., Akao, S.
- Plant cell reports 2004 v.22 no.11 pp. 848-858
- Oryza sativa, rice, in vitro regeneration, micropropagation, cell suspension culture, cultured cells, proteome, plant proteins, aldehyde oxidoreductases, messenger RNA, culture media, auxins, 2,4-D, zinc, proteomics, amino acid sequences, nucleotide sequences, two-dimensional gel electrophoresis
- Proteins induced in rice by auxin and zinc were determined by proteome analysis. Cultured suspension cells of rice were treated with 2,4-dichlorophenoxyacetic acid and ZnSO4 and then proteins were separated by two-dimensional polyacrylamide gel electrophoresis; seven proteins were found to be induced by auxin and zinc. Of these seven, methylmalonate-semialdehyde dehydrogenase (MMSDH) was elevated by treatment with auxin alone. MMSDH was detected in cultured suspension cells, root and leaf sheath, but not in leaf blades. MMSDH responded to auxin and gibberellin, but did not respond to brassinolide and cytokinin. Furthermore, the amount of MMSDH in slr1, a constitutive gibberellin response mutant, was 2-fold that of wild type. MMSDH mRNA and protein were stimulated in root formation induced by auxin and/or zinc over a 4-week period. These results suggest that MMSDH may be necessary for root formation in rice induced by auxin and/or zinc.