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A reliable method for extraction of RNA from various conifer tissues

Author:
Dong, J.Z., Dunstan, D.I.
Source:
Plant cell reports 1996 v.15 no.7 pp. 516-521
ISSN:
0721-7714
Subject:
Arabidopsis thaliana, biosynthesis, quantitative analysis, stems, embryo (plant), DNA, clones, conifer needles, Picea glauca, Nicotiana tabacum, gene expression, genetic techniques and protocols, RNA, roots, cell suspension culture
Abstract:
A simple and efficient procedure suitable for extraction of high quality RNA from cultured conifer tissues, somatic embryos, zygotic embryos, needles, stem and root tissues was developed. It produced from 100 microgram up to 700 microgram total RNA per gram tissue dependent on the types of tissues used. RNA quality was estimated by spectrophotometry, agarose gel electrophoresis, in vitro translation of mRNA, cDNA synthesis and Northern blot analysis. The method also worked well with Arabidopsis thaliana and tobacco tissues.
Agid:
1436631