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Effect of promoter-leader sequences on transient expression of reporter gene chimerase biolistically transferred into sugarbeet (Beta vulgaris) suspension cells
- Ingersoll, J.C., Heutte, T.M., Owens, L.D.
- Plant cell reports 1996 v.15 no.11 pp. 836-840
- Beta vulgaris, cell suspension culture, reporter genes, biolistics, gene transfer, chimerism, gene expression, beta-glucuronidase, enzyme activity, promoter regions
- Chimeric constructs consisting of the gus coding region fused downstream of promoter-untranslated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells. Each construct was expressed in recipient cells at 6 h after bombardment with maximum levels observed between 12 and 48 h. Expression of the PR-S construct mimicked the time-course expression of the constitutively expressed 35S construct but reached levels almost 50% higher. The pin2-promoter construct was ultimately expressed at levels similar to that of PR-S. Expression of the osmotin promoter-leader construct was highest, reaching levels approximately 2.5-fold higher than those of the 35S construct.