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Somatic embryogenesis and regeneration of triploid plants in endosperm cultures of Acacia nilotica

Author:
Garg, L., Bhandari, N.N., Rani, V., Bhojwani, S.S.
Source:
Plant cell reports 1996 v.15 no.11 pp. 855-858
ISSN:
0721-7714
Subject:
Acacia nilotica, endosperm, cultured cells, culture media, 2,4-D, benzyladenine, glutamine, coconuts, plant extracts, dose response, callus, cell differentiation, embryo (plant), germination, somatic embryogenesis, triploidy, plant morphology, developmental stages
Abstract:
Immature endosperm of Acacia nilotica formed a nodular callus on MS medium supplemented with 2,4-D, BAP and CH. In the third passage on this medium, in the dark the callus differentiated somatic embryos. The embryos germinated on MS only after 15 d pre-treatment on modified MS medium in which major salts were replaced by those of major salts of B5 medium and supplemented with glutamine CH and CW. Triploid nature of the somatic embryos was confirmed by Feulgen cytophotometry.
Agid:
1436733