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Regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (Gossypium hirsutum L.)

Author:
Rajasekaran, K.
Source:
Plant cell reports 1996 v.15 no.11 pp. 859-864
ISSN:
0721-7714
Subject:
Gossypium hirsutum, cell suspension culture, cryopreservation, callus, cultivars, germplasm, herbicide resistance, insect pests, pest resistance, cryoprotectants, dimethyl sulfoxide, dose response, methodology, plant morphology
Abstract:
Successful regeneration of cotton (Gossypium hirsutum L.) plants from cryopreserved embryogenic callus and cell suspension cultures is described. The cryoprotectant mixture consisting of a modified Murashige and Skoog (1962) medium with sucrose (5% w/v), DMSO (5% v/v) and glycerol (5% v/v) gave the highest survival rate (70%) from cell suspension cultures cryopreserved in liquid nitrogen after slow cooling (0.5 to 1.0 degrees C/min). A cooling rate of 0.5 degrees C/min provided a satisfactory recovery rate (30%) from cryopreserved embryogenic callus cultures and was superior to a cooling rate of 1 degrees C/min. Regenerated plants from cell suspension and embryogenic callus cultures cryopreserved for more than four years exhibited normal morphology, growth and boll set upon transfer to soil.
Agid:
1436735