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Regeneration of plants from cryopreserved embryogenic cell suspension and callus cultures of cotton (Gossypium hirsutum L.)
- Rajasekaran, K.
- Plant cell reports 1996 v.15 no.11 pp. 859-864
- germplasm, cultivars, insect pests, dose response, cryopreservation, Gossypium hirsutum, dimethyl sulfoxide, cryoprotectants, herbicide resistance, plant morphology, pest resistance, cell suspension culture, callus
- Successful regeneration of cotton (Gossypium hirsutum L.) plants from cryopreserved embryogenic callus and cell suspension cultures is described. The cryoprotectant mixture consisting of a modified Murashige and Skoog (1962) medium with sucrose (5% w/v), DMSO (5% v/v) and glycerol (5% v/v) gave the highest survival rate (70%) from cell suspension cultures cryopreserved in liquid nitrogen after slow cooling (0.5 to 1.0 degrees C/min). A cooling rate of 0.5 degrees C/min provided a satisfactory recovery rate (30%) from cryopreserved embryogenic callus cultures and was superior to a cooling rate of 1 degrees C/min. Regenerated plants from cell suspension and embryogenic callus cultures cryopreserved for more than four years exhibited normal morphology, growth and boll set upon transfer to soil.