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Phenylacetic acid induced organogenesis in cultured leaf segments of Dianthus chinensis
- Jethwani, V., Kothari, S.L.
- Plant cell reports 1996 v.15 no.11 pp. 869-872
- Dianthus chinensis, leaves, cell culture, organogenesis, cultured cells, phenylacetic acid, shoots, callus, culture media, 2,4-D, benzyladenine, dose response, micropropagation, naphthaleneacetic acid
- Shoot regeneration was achieved from leaf derived callus of Dianthus chinensis using Phenylacetic acid (PAA). Callus from basal leaf segments, raised on Murashige and Skoog's (MS) medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) or 1-Naphthaleneacetic acid (NAA) in combination with 6-benzylamino purine (BAP), was subcultured on medium supplemented with BAP in combination with 2,4-D, NAA or PAA. Shoots were induced only when leaf derived callus was subcultured on medium containing BAP (2.0, 5.0 mg/l) in combination with PAA (0.5, 1.0 mg/l). No shoot regeneration was observed when 2,4-D, NAA or BAP were used in the medium either singly or in different combinations. These results demonstrate that PAA in combination with BAP was essential to trigger shoot regeneration from cultured leaf callus of D. chinensis.