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Cryopreservation of non-encapsulated embryogenic tissue of sweet potato (Ipomoea batatas)
- Blakesley, D., Al Mazrooei, S., Bhatti, M.H., Henshaw, G.G.
- Plant cell reports 1996 v.15 no.11 pp. 873-876
- Ipomoea batatas, germplasm, cryopreservation, embryo (plant), culture media, 2,4-D, benzyladenine, methodology, sucrose, encapsulation, dose response, tissue culture, shoots
- Embryogenic tissue of the sweet potato (Ipomoea batatas (L) LAM) genotype TIB 10 was established from in vitro axillary shoot tips on Murashige and Skoog (1962) medium supplemented with 5 micromolars 2,4-dichlorophenoxyacetic acid. Embryogenic aggregates of fresh mass 9.0-12 mg were subjected to a rapid freezing protocol in liquid nitrogen following sucrose preculture and varying degrees of dehydration. Up to 50% of embryogenic explants survived rapid freezing after preculture on 0.4 or 0.7M sucrose only. Dehydration with silica gel to moisture contents in the range 18-41% improved the survival after cryopreservation of embryogenic tissue. Tissue dehydrated for intermediate periods exhibited poor survival. Following freezing, embryogenic tissue appeared to develop normally, retaining its competence to produce mature embryos and plantlets.