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Effect of cadimum treatment on the expression of chimeric genes in transgenic tobacco seedlings and calli
- Stefanov, I., Frank, J., Gedamu, L., Misra, S.
- Plant cell reports 1997 v.16 no.5 pp. 291-294
- Nicotiana tabacum, cadmium, dose response, phytotoxicity, plant breeding, chromosome mapping, gene transfer, transgenic plants, chimerism, reporter genes, gene expression, seedlings, callus, beta-glucuronidase, enzyme activity, measurement, glucuronic acid, transcription (genetics), promoter regions
- Transgenic tobacco plants and calli bearing the bacterial uidA A gene under the transcriptional control of rbcS, mas and CaMV35S promoter(s) were exposed to different concentrations of cadmium. The transcriptional activity of the promoters was monitored using p-nitrophenyl beta-D-glucuronide as a substrate for the beta-glucuronidase (uidA) reporter enzyme. The rbcS promoter was repressed by high concentrations of cadmium. An induction of the mas promoter was seen after cadmium treatment of seedling, but not calli. The activity of the CaMV35S promoter was by cadmium in both seedlings and calli.