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Agrobacterium strain specificity and shooty tumour formation in eucalypt (Eucalyptus grandis X E. urophylla)

Machado, L. de O.R., Andrade, G.M. de., Barrueto Cid, L.P., Penchel, R.M., Brasileiro, A.C.M.
Plant cell reports 1997 v.16 no.5 pp. 299-303
Eucalyptus grandis, Eucalyptus urophylla, genetic transformation, Agrobacterium tumefaciens, Rhizobium rhizogenes, gene transfer, methodology, pathogenicity, strain differences, neoplasms
To develop a successful protocol for Agrobacterium-mediated transformation in plants it is essential to determine the most efficient bacterial strain/plant genotype interaction. In the present work, we evaluated the susceptibility of Eucalyptus grandis x E. urophylla to five Agrobacterium rhizogenes and twelve A. tumefaciens wild-type strains. The results showed different degrees of virulence, according to the strain tested, indicating that transformation of this eucalypt hybrid by Agrobacterium-derived vectors is possible. All developed tumours showed an autonomous growth when transferred to a hormone-free medium. Some of these tumours formed shoots spontaneously, with a normal phenotype. Polymerase Chain Reaction (PCR) and Southern blot analyses were performed to confirm the absence of the oncogenic T-DNA in plants derived from these shooty tumours.