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Pisatin metabolism in pea (Pisum sativum L.) cell suspension cultures

Borejsza-Wysocki, W., Borejsza-Wysocka, E., Hrazdina, G.
Plant cell reports 1997 v.16 no.5 pp. 304-309
Pisum sativum, cell suspension culture, pisatin, metabolism, biosynthesis, quantitative analysis, chemical reactions, biochemical pathways, phenylalanine ammonia-lyase, oxidoreductases, enzyme activity, plant pathogens, defense mechanisms, disease resistance, naringenin-chalcone synthase
Cell suspension cultures were established from germinating pea (Pisum sativum L.) seeds. This cell culture, which accumulated pisatin, consisted mostly of single cells containing a few cell aggregates. The cells responded to treatment with a yeast glucan preparation with transient accumulation of pisatin in both cells and culture media. Addition of pisatin to cell cultures resulted in increased synthesis of pisatin. Phenylalanine ammonia-lyase, chalcone synthase and isoflavone reductase activities were present in untreated cells. Upon treatment with an elicitor preparation the activities of the first two enzymes showed a rapid, transient increase up to 20 hours after treatment. Isoflavone reductase showed a major and minor peak at 16 and 36 h, respectively, after elicitor treatment. The time course of the enzyme activity and pisatin accumulation is consistent with an elicitor-mediated response.