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Variability in storage response within a coffee (Coffea spp.) core collection under slow growth conditions

Dussert, S., Chabrillange, N., Anthony, F., Engelmann, F., Recalt, C., Hamon, S.
Plant cell reports 1997 v.16 no.5 pp. 344-348
Coffea, germplasm, shelf life, culture media, methodology
An in vitro core collection of African coffee germplasm, structured in 32 diploid diversity groups, was established and conserved under slow growth for 3 years (6 subcultures). The initial objective was to store twenty accessions per group, with four replicates per accession. A statistical model was developed to analyse observations of survival rates within each diversity group. The goodness of fit of the model was shown. Survival analysis indicated a broad variability of the accessions in their response to the storage conditions and confirmed the importance of structuring the coffee complex down to the intraspecific level. Intra- and inter-group differences had consequences on the genetic representativity of the in vitro core collection. For practical purposes, conservation was carried on when the intra-group genetic drift was less than 50%.