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Variability in storage response within a coffee (Coffea spp.) core collection under slow growth conditions
- Dussert, S., Chabrillange, N., Anthony, F., Engelmann, F., Recalt, C., Hamon, S.
- Plant cell reports 1997 v.16 no.5 pp. 344-348
- Coffea, germplasm, shelf life, culture media, methodology
- An in vitro core collection of African coffee germplasm, structured in 32 diploid diversity groups, was established and conserved under slow growth for 3 years (6 subcultures). The initial objective was to store twenty accessions per group, with four replicates per accession. A statistical model was developed to analyse observations of survival rates within each diversity group. The goodness of fit of the model was shown. Survival analysis indicated a broad variability of the accessions in their response to the storage conditions and confirmed the importance of structuring the coffee complex down to the intraspecific level. Intra- and inter-group differences had consequences on the genetic representativity of the in vitro core collection. For practical purposes, conservation was carried on when the intra-group genetic drift was less than 50%.