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Analyses in transgenic tobacco of the promoter from a Brassica napus gene highly expressed in the stigma

Author:
Robert, L.S., Levesque-Lemay, M., Gerster, J.L., Hong, H.P., Keller, W.
Source:
Plant cell reports 1999 v.18 no.5 pp. 357-362
ISSN:
0721-7714
Subject:
Arabidopsis thaliana, genetic transformation, gene transfer, transgenic plants, Agrobacterium radiobacter, developmental stages, clones, gene expression, stigma, amino acid sequences, Nicotiana tabacum, genes, Brassica napus, nucleotide sequences, enzyme activity, histochemistry, beta-glucuronidase
Abstract:
A genomic clone, Pis G363, containing the Brassica napus stigma-expressed gene Pis 63-2 was isolated and sequenced. The coding region of Pis G363 does not possess introns and shows 82% identity to the nucleotide sequence of a gene from Arabidopsis BAC clone T01B08. A 2-kb promoter fragment from Pis G363 was fused to the coding sequence of the marker enzyme beta-glucuronidase (GUS) and introduced into tobacco via Agro-bacterium-mediated transformation. The promoter fragment directed expression of the GUS gene in the stigma of transgenic tobacco. Some transformants also showed relatively low GUS activity in the pollen.
Agid:
1436987