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Regeneration and large-scale propagation of bamboo (Dendrocalamus strictus Nees) through somatic embryogenesis
- Saxena, S., Dhawan, V.
- Plant cell reports 1999 v.18 no.5 pp. 438-443
- Dendrocalamus strictus, somatic embryogenesis, shoots, callus, culture media, 2,4-D, abscisic acid, benzyladenine, kinetin, dose response, developmental stages, plant morphology, micropropagation, methodology, industrial applications, naphthaleneacetic acid, indole acetic acid, indole butyric acid, India
- A complete protocol for large-scale propagation of Dendrocalamus strictus Nees by somatic embryo-genesis has been developed. Seeds cultured on agar-solidified Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D; 3xl0-5 M) produced embryogenic callus from proliferation of the embryo. Somatic embryos formed in vitro multiplied rapidly (two- to five fold every 5 weeks) on semi-solid MS medium containing 2,4-D (1x10-5 M), kinetin (Kn) (5X10-6 M), 1-indolebutyric acid (IBA) (2x10-6 M) and soluble polyvinylpyrrolidone (PVP) (250 mg l-1), or MS with 2,4-D (1x10-5 M), 6-benzylaminopurine (BAP) (1x10-5 M), and soluble PVP (250 mg l-1). Upon transfer to MS containing 1-naphthaleneacetic acid (NAA) (5x10-6 M), Kn (5x10-6 M) and soluble PVP (250 mg l-1), the dark-green embryos developed into healthy plantlets. Unrooted shoots, if any, obtained on the multiplication media were rooted on MS major salts reduced to half strength supplemented with NAA (3x10-6 M) and IBA (2.5x10-6 M). The rooted plants were successfully transferred to soil in polythene bags with over 80% survival. Using this methodology, more than 100,000 plants have been produced.