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Cryopreservation of in vitro-grown shoot-tips of hop (Humulus lupulus L.) using encapsulation/dehydration
- Martinez, D., Tames, R.S., Revilla, M.A.
- Plant cell reports 1999 v.19 no.1 pp. 59-63
- Humulus lupulus, germplasm, cryopreservation, encapsulation, methodology, culture media, alginates, sucrose, benzyladenine, gibberellic acid, dose response, developmental stages, plant morphology, tissue culture, shoots, indole butyric acid
- A cryopreservation procedure using encapsulation/dehydration was established for shoot-tips obtained from in vitro-grown shoots of hop. After dissection, shoot-tips were encapsulated in medium with alginate and 0.5 M sucrose. Optimal conditions consisted of preculture for 2 days in solid medium with 0.75 M sucrose, or in increasing sucrose concentrations, desiccation for 4 h with silicagel in a flow cabinet (16% water content) followed by rapid freezing and slow thawing. Shoot recovery after freezing 60 min in liquid nitrogen was around 80%. No phenotypical changes were observed in the recovered plants from cryopreserved shoot-tips growing in the field.