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Sex pheromone biosynthesis in the tortricid moth, Ctenopseustis herana (Felder & Rogenhofer)

Author:
Foster, S.P., Roelofs, W.L.
Source:
Archives of insect biochemistry and physiology 1996 v.33 no.2 pp. 135-147
ISSN:
0739-4462
Subject:
Ctenopseustis, sex pheromones, biosynthesis, chemical composition, fatty acids, esters, isotope labeling, myristic acid, chemical reactions, decapitation, neuropeptides, hormonal regulation, animal glands, pheromone biosynthesis activating neuropeptide
Abstract:
Biosynthesis of the unusual sex pheromone component (Z)-5-tetradecenyl acetate (Z5-14:OAc) was studied in the tortricid moth, Ctenopseustis herana (Felder & Rogenhofer), by fatty acid methyl ester (FAME) analysis of the pheromone gland, and topical application of various deuterium-labelled acids to the gland. The only unusual unsaturated FAME found in base-methanolyzed glands of females was the putative precursor of the pheromone, methyl (Z)-5-tetradecenoate (Z5-14:Me). When deuterium-labelled, myristic, palmitic, stearic, lauric, and oleic acids were applied to the gland, label was incorporated into the pheromone component only from the first three acids (i.e., Z5-14:OAc could not be biosynthesized from lauric or oleic acids). Furthermore, the amount of label incorporated from the first three acids decreased in the order myristic > palmitic > stearic. Application of labelled myristic acid to the gland resulted in incorporation of label into Z5-14:Me. These data are consistent with the biosynthesis of Z5-14:OAc in C. herana resulting from delta 5-desaturation of myristic acid, a novel biosynthetic route for a moth sex pheromone component. Regulation of pheromone biosynthesis in this species was also investigated. Decapitation of female C. herana resulted in a significant decline in sex pheromone titre. Injection of female head extract or synthetic Bombyx mori pheromone biosynthesis-activating neuropeptide (PBAN) into decapitated female C. herana gave a significant increase in pheromone titre, suggesting that pheromone biosynthesis in this species is regulated by a PBAN or PBAN-like substance.
Agid:
1438805