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A rapid antibody-based test for Sec-2, a marker for the short arm of chromosome 2 of rye (2RS)
- Skerritt, J.H., Andrews, J.L., Stoddard, F.L., Gupta, R.B., Howes, N.K.
- Genome = 1996 v.39 no.5 pp. 1006-1012
- substitution lines, intergeneric hybridization, loci, seeds, translocation lines, prolamins, Secale cereale, chromosome substitution, monoclonal antibodies, enzyme-linked immunosorbent assay, Triticum aestivum, triticale, chromosome translocation, screening, detection, introgression
- A simple monoclonal antibody-based screening test has been developed for the presence of translocations of the short arm of chromosome 2 of rye (2RS) with wheat chromosome 2B. 2RS encodes a set of about three polypeptides known as Mr 75000 gamma-secalins. Use of the antibody test for these secalins enabled screening of several hundred seeds per day. The antibody could readily distinguish 1BL-1RS translocations and 2R substitutions from 1BL-1RS translocations or nontranslocation wheats. Use of the antibody in analysis of segregating progeny for Sec-2 in several wheat backgrounds was successful. Results with a selection of the seed population were checked using protein gel electrophoresis, with 100% correct confirmation.