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Molecular genetic mapping of three X-linked avirulence genes, vH6, vH9 and vH13, in the Hessian fly

Schulte, S.J., Rider, S.D. Jr., Hatchett, J.H., Stuart, J.J.
Genome 1999 v.42 no.5 pp. 821-828
Mayetiola destructor, chromosome mapping, molecular genetics, virulence, genes, pest resistance, Triticum aestivum, genetic resistance, genetic markers, genetic distance, nucleotide sequences, X chromosome, linkage (genetics), nucleic acid hybridization, phenotype, frequency
Three X-linked avirulence genes, vH6, and vH9, and vH13 in the Hessian fly, Mayetiola destructor, confer avirulence to Hessian fly resistance genes H6, H9, and H13 in wheat. We used a combination of two- and three-point crosses to determine the order of these genes with respect to each other, the white eye mutation and three X-linked molecular markers, G15-1, 020, and 021, developed from genomic lambda clones, lambdaG15-1, lambda020, and lambda021. The gene order was determined to be vH9-vH6-G15-1-w-vH13-020-021. In situ hybridization of lambdaG15-1, lambda020, and lambda021, on the polytene chromosomes of the Hessian fly salivary gland established their orientation on Hessian fly chromosome X1. Based on the size of the Hessian fly genome, and the genetic distances between markers, the relationship of physical to genetic distance was estimated at no more than 300 kb/cM along Hessian fly chromosome X1, suggesting that map-based cloning of these avirulence genes will be feasible.