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Production of beta-Fructofuranosidase from Sporotrichum thermophile and its application in the synthesis of fructooligosaccharides
- Katapodis, P., Kalogeris, E., Kekos, D., Macris, B.J., Christakopoulos, P.
- Food biotechnology 2003 v.17 no.1 pp. 1-14
- Myceliophthora thermophila, thermophilic fungi, biological production, beta-fructofuranosidase, culture media, enzyme substrates, organic nitrogen compounds, inorganic compounds, carbon, salt concentration, pH, temperature, inoculum, microbial resuscitation, metal ions, glycosylation, enzyme activity, enzymatic hydrolysis, kestose, fructooligosaccharides
- Production of beta-fructofuranosidase from the thermophilic fungus Sporotrichum thermophile was studied. The effect of nitrogen source, as well as the type and concentration of carbon source on enzyme production was examined. The results from flask experiments were used for the production of the enzyme in 7-1 bioreactors. beta-Fructofuranosidase from Sporotrichum thermophile showed both transfructosylating and hydrolytic activities. It was optimally active at 60 degrees C, while the optimal pHs for hydrolysis and transfructosylation were 4.0 and 6.0, respectively. Synthesis of fructooligosaccharides was maximized at 20% (w/v) initial sucrose concentration. The major sugar produced by the transfructosylating activity of the enzyme was 6-kestose.