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Development and validation of a method for the quantitative determination of aflatoxin contaminants in Maytenus ilicifolia by HPLC with fluorescence detection

Author:
Braga, S.M.L.F.M., Medeiros, F. D. de, Oliveira, E. de J., Macedo, R.O.
Source:
Phytochemical analysis 2005 v.16 no.4 pp. 267-271
ISSN:
0958-0344
Subject:
Maytenus, medicinal plants, herbaceous plants, aflatoxins, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, quantitative analysis, high performance liquid chromatography
Abstract:
A method for the quantification of aflatoxins B1, G1, B2 and G2 in the medicinal herb Maytenus ilicifolia was developed and validated. The method used immunoaffinity columns for sample clean-up and HPLC with fluorescence detection without any derivatisation step. The method showed good inter-day accuracy (bias values in the range 4.5-10.7%) and precision (5-16% RSD) when applied to the determination of levels of aflatoxins ranging from 7 to 20 ppb in the plant material. The detection limits for samples of the plant material spiked with aflatoxins were 3.5 ng/g for B1 and G1 and 0.1 ng/g for B2 and G2. The method was successfully applied to commercial samples of Maytenus ilicifolia for the screening of aflatoxin contaminants.
Agid:
1446113