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Development and validation of a method for the quantitative determination of aflatoxin contaminants in Maytenus ilicifolia by HPLC with fluorescence detection

Braga, S.M.L.F.M., Medeiros, F. D. de, Oliveira, E. de J., Macedo, R.O.
Phytochemical analysis 2005 v.16 no.4 pp. 267-271
Maytenus, medicinal plants, herbaceous plants, aflatoxins, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, quantitative analysis, high performance liquid chromatography
A method for the quantification of aflatoxins B1, G1, B2 and G2 in the medicinal herb Maytenus ilicifolia was developed and validated. The method used immunoaffinity columns for sample clean-up and HPLC with fluorescence detection without any derivatisation step. The method showed good inter-day accuracy (bias values in the range 4.5-10.7%) and precision (5-16% RSD) when applied to the determination of levels of aflatoxins ranging from 7 to 20 ppb in the plant material. The detection limits for samples of the plant material spiked with aflatoxins were 3.5 ng/g for B1 and G1 and 0.1 ng/g for B2 and G2. The method was successfully applied to commercial samples of Maytenus ilicifolia for the screening of aflatoxin contaminants.