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Cyclopentenone isoprostanes induced by reactive oxygen species trigger defense gene activation and phytoalexin accumulation in plants

Thoma, I., Loeffler, C., Sinha, A.K., Gupta, M., Krischke, M., Steffan, B., Roitsch, T., Mueller, M.J.
Plant journal 2003 v.34 no.3 pp. 363-375
scopoletin, signal transduction, plant pathogenic fungi, oxidative stress, phenylalanine ammonia-lyase, Botrytis cinerea, glutathione transferase, gene activation, jasmonic acid, Tilia cordata, Salix alba, Nicotiana tabacum, Arabidopsis thaliana, peroxides, bioaccumulation, Betula pendula, beta-fructofuranosidase, culture media, tobacco, Solanum lycopersicum var. lycopersicum, biochemical pathways, cultured cells, Rauvolfia serpentina, phytoalexins
Lipid peroxidation may be initiated either by lipoxygenases or by reactive oxygen species (ROS). Enzymatic oxidation of α-linolenate can result in the biosynthesis of cyclic oxylipins of the jasmonate type while free-radical-catalyzed oxidation of α-linolenate may yield several classes of cyclic oxylipins termed phytoprostanes in vivo. Previously, we have shown that one of these classes, the E1-phytoprostanes (PPE1), occurs ubiquitously in plants. In this work, it is shown that PPE1 are converted to novel cyclopentenone A1- and B1-phytoprostanes (PPA1 and PPB1) in planta. Enhanced formation of PPE1, PPA1, and PPB1 is observed after peroxide stress in tobacco cell cultures as well as after infection of tomato plants with a necrotrophic fungus, Botrytis cinerea. PPA1 and PPB1 display powerful biologic activities including activation of mitogen-activated protein kinase (MAPK) and induction of glutathione-S-transferase (GST), defense genes, and phytoalexins. Data collected so far infer that enhanced phytoprostane formation is a general consequence of oxidative stress in plants. We propose that phytoprostanes are components of an oxidant-injury-sensing, archaic signaling system that serves to induce several plant defense mechanisms.