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Somatic and germinal activities of maize Activator (Ac) transposase mutants in transgenic tobacco
- Kunze, R., Kuhn, S., Jones, J.D.G., Scofield, S.R.
- The plant journal 1995 v.8 no.1 pp. 45-54
- crossing, Nicotiana tabacum, Petunia hybrida, transposons, recombinant DNA, plasmid vectors, transgenic plants, genetic transformation, dissociation, streptomycin, seedlings, reporter genes, epistasis, gene expression, site-directed mutagenesis
- The properties have been investigated of two deletion derivatives of the transposase protein (TPase) of maize transposable element Ac in transgenic tobacco. The wildtype and mutant TPases were expressed as fusions to the cauliflower mosaic virus 35S promoter. A deletion of 102 amino acids from the N-terminus, TPase(103-807), induces Ds excisions from a SPT::Ds reporter locus with a higher frequency than the wild-type TPase. The increased transpositional activity of TPase(103-807) is a dominant trait, as seedlings coexpressing truncated and wild-type TPase show the characteristic TPase(103-807) variegation phenotype. A transpositionally inactive TPase deletion derivative lacking 188 amino acids from the N-terminus inhibits the transpositional activity of the wild-type TPase.