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Multiple photoreceptors mediate the light-induced reduction of GUS-COP1 from Arabidopsis hypocotyl nuclei

Osterlund, M.T., Deng, X.W.
The plant journal 1998 v.16 no.2 pp. 201-208
Arabidopsis thaliana, hypocotyls, mutants, beta-glucuronidase, plant proteins, photoreceptors, light, seedlings, transgenic plants, gene expression, phytochrome, cytochemistry, far-red light, red light, blue light, plant development, reporter genes, binding proteins, recombinant proteins, histochemistry
The subcellular localization of COP1, a key photomorphogenic repressor, is regulated by light in Arabidopsis seedlings. Photoreceptor loss-of-function mutants and dominant gain-of-function overexpression transgenes were both used to analyze the influences of the three photoreceptors, phyA, phyB, and CRY1, on the light-regulated subcellular localization of COP1. Through a semiquantitative analysis of the nuclear abundance of GUS-COP1 in the various genetic backgrounds, the specific roles of the individual photoreceptors have been established. The data suggest that multiple photoreceptors influence the light-regulated subcellular localization of COP1 in white light. Under specific wavelengths of light, phyA, phyB, and CRY1 each play critical roles in mediating far-red, red, and blue light signals, respectively. Our data also support an interdependency between CRY1 and the phytochromes in mediating the light-regulated subcellular localization of COP1 and thus seedling development.