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PCR primers for the amplification of four insect mitochondrial gene fragments
- Kambhampati, S., Smith, P.T.
- Insect molecular biology 1995 v.4 no.4 pp. 233-236
- insects, mitochondrial DNA, polymerase chain reaction, genes, sequence analysis, nucleotide sequences, ribosomal DNA, ribosomal RNA, NADH dehydrogenase, cytochrome-c oxidase, genomics, DNA primers
- Insect mitochondrial genome (mtDNA) analysis is a powerful tool for the study of population genetics and phylogenetics. In the past few years primer sequences for the PCR amplification of various insect mtDNA genes have been published. The objectives of this study were (1) present new primer sequences for six insect mitochondrial genes and (2) test primers designed in our laboratory and some previously published primers on a wide range of insects to determine if amplification of the target fragment could be obtained. The primers for the amplification of the two ribosomal RNA gene (16S and 12S rRNA) fragments are universal for insects and related groups; the primers for NADH5 and NADH4 dehydrogenase gene fragments and cytochrome c oxidase I gene fragment are applicable broadly.