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Feeding juvenile Artemia enriched with ascorbic acid improves larval survival in the spiny lobster Jasus edwardsii

Smith, G.G., Brown, M.R., Ritar, A.J.
Aquaculture nutrition 2004 v.10 no.2 pp. 105-112
crustacean culture, animal growth, Jasus edwardsii, juveniles, animal stress, Artemia, polyphosphates, animal feeding, ascorbic acid, lobsters, ecdysis, mortality, larvae
Newly hatched Jasus edwardsii phyllosoma were fed unenriched Artemia endogenous ascorbic acid (AA) concentration of 166 microgram g(-1) dry weight (dw), Artemia supplemented with algae (AA concentration 594 microgram g(-1) dw) or with ascorbyl-2-polyphosphate (A2P) (AA concentration 11 737 microgram g(-1) dw) to examine possible benefits of AA enhancement on culture. Plain or algal-enriched Artemia were fed continuously for 28 days in two treatments during the study. Four other treatments received A2P-enriched Artemia on a progressive basis starting from the commencement of the trial (D-0), the third (D-3), sixth (D-6) or ninth day (D-9) of Stage I (14 days) and similarly during Stage II (14 days). Prior to the commencement of A2P supplementation, plain Artemia were supplied to these animals. By Stage III (28 days feeding), algal, D-0 and D-3 phyllosoma had attained the largest size. The uptake and retention of AA by Stage III phyllosoma appeared to be dose-dependent with the highest concentration of AA incorporation evident in D-0 phyllosoma (1816 microgram g(-1) dw), while algal and plain phyllosoma contained the lowest concentrations (600 and 300 microgram g(-1) dw, respectively). Survival at Stage III was highest in D-0 phyllosoma (89%) and lowest in plain phyllosoma (51%). There was a positive relationship between phyllosoma AA concentration and larval survival (R2 = 0.8328, P < 0.0001). D-0 phyllosoma had the lowest stress index when subjected to an osmotic/temperature activity test, indicative of better survival in culture compared to plain, algal and D-9 phyllosoma, which had consistently higher indices. A negative relationship existed between phyllosoma AA concentration and stress indices at Stage III (R2 = 0.9263, P < 0.0001), suggesting that AA from the Artemia diet conferred stress resistance.