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The isolation and recovery of fatty acids with delta 5 unsaturation from meadowfoam oil by lipase-catalyzed hydrolysis and esterification
- Hayes, D.G., Kleiman, R.
- Journal of the American Oil Chemists' Society 1993 v.70 no.6 pp. 555
- Limnanthes alba, seed oils, hydrolysis, esterification, triacylglycerol lipase, unsaturated fatty acids
- This report examines the use of lipases for isolating fatty acids with Δ5 unsaturation from the seed oil ofLimnanthes alba, or meadowfoam. Seven lipase types and three enzyme configurations (immobilized, “free” and reversemicellar encapsulated) were examined. All lipases discriminated against Δ5 acids to varying degrees, but the degree of discrimination was independent of enzyme configuration. Lipase-catalyzed esterification of meadowfoam oil's free fatty acids was much more successful for isolating Δ5 acyl groups than was lipolysis. For example, esterification directed byChromobacterium viscosum lipase yielded a free fatty acid product containing >95% of the Δ5 acyl groups at >99% purity.