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Seroepidemiological survey of Leishmania infantum infection in dogs from northeastern Portugal

Author:
Sousa, Susana, Lopes, Ana Patricia, Cardoso, Luís, Silvestre, Ricardo, Schallig, Henk, Reed, Steven G., Cordeiro da Silva, Anabela
Source:
Acta tropica 2011 v.120 no.1-2 pp. 82-87
ISSN:
0001-706X
Subject:
serological surveys, blood serum, antigens, seroprevalence, dogs, agglutination tests, risk factors, leishmaniasis, Leishmania infantum, enzyme-linked immunosorbent assay, odds ratio, regression analysis, Toxoplasma gondii, habitats, adults, Portugal
Abstract:
Northeastern Portugal is a region where canine leishmaniasis (CanL) is endemic. In this study, a sero-epidemiological survey was conducted in 654 dogs from that geographical area. Serum samples were evaluated by the direct agglutination test (DAT) and also by enzyme-linked immunosorbent assay (ELISA) using five different defined antigens. Seroprevalence of infection was 21.3% based on the assumption that seropositive animals were positive for at least three tests. A high degree of agreement was found between DAT and LAM-ELISA (89%; kappa value [κ]=0.67). A statistically significant difference (p<0.05) of seropositivity was found between adult (23.4%) and juvenile dogs (12.2%), apparently healthy (14.8%) and sick dogs (40.2%), vaccinated (19.7%) and non-vaccinated (41.2%) animals, seropositive (26.9%) and seronegative (18.0%) for Toxoplasma gondii, living in rural (18.5%) or urban (32.6%) areas, and between animals living exclusively outdoors (18.2%) and those living in a mixed habitat (27.5%). Risk factors for canine Leishmania infection, as defined by multiple logistic regression analysis, were of clinical status (odds ratio [OR]=3.1) and Toxoplasma infection (OR=1.5).
Agid:
146283