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Gemmation in cultured gametophytes of Osmunda regalis

Author:
Fernandez, H., Bertrand, A.M., Sanchez-Tames, R.
Source:
Plant cell reports 1997 v.16 no.5 pp. 358-362
ISSN:
0721-7714
Subject:
Osmunda regalis, germ cells, in vitro culture, methodology, culture media, sugars, dose response, pH, light intensity, cell growth, plant development, micropropagation, developmental stages, plant anatomy
Abstract:
Gametophytes of Osmunda regalis were cultured in vitro to determine the optimal conditions for growth and development. Culture media with low ammonium concentration (Knop, Knudson or 1/4 Murashige and Skoog dilution) were the most effective for growth of this organism. Addition of sucrose and mannitol to the culture medium at the same osmoticum, inhibits or enhances, respectively, growth and development of the gametophytes, which show a strong autotrophy in vitro. In darkness, both two-dimensional growth and sexual organ formation took place. Gemmae formation is described for the first time in this species and this process does not occur without sucrose in the culture medium or in darkness.
Agid:
1470256