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Effect of acetylsalicylic acid on secondary metabolism of Catharanthus roseus tumor suspension cultures
- Godoy-Hernandez, G., Loyola-Vargas, V.M.
- Plant cell reports 1997 v.16 no.5 pp. 287-290
- Catharanthus roseus, neoplasms, cell lines, cell suspension culture, metabolism, aspirin, dose response, Aspergillus fumigatus, cinnamic acid, secondary metabolites, biosynthesis, alkaloids, phenolic compounds, furans, coumarins, quantitative analysis, anthocyanins, culture media, carbon, corn starch
- Addition of various concentrations (0.5-20 mM) of acetylsalicylic acid (ASA) to tumor lines of Catharanthus roseus cultivated in vitro and requiring corn starch as carbon source, produced remarkable effects on secondary metabolite production. An increase of 505% total alkaloids per culture (cells plus liquid medium), 1587% total phenolics (liquid medium), 612% total furanocoumarins (liquid medium), and 1476% total anthocyanins (liquid medium) was detected. 1 mM ASA in combination with other elicitors, such as homogenates of Aspergillus fumigatus or trans-cinnamic acid, did not further increase the metabolite content substantially. The results suggest that ASA could act as a new biotic elicitor of metabolite production in C. roseus cell suspension culture.