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Trichoderma harzianum expressed sequence tags for identification of genes with putative roles in mycoparasitism against Fusarium solani

Author:
Steindorff, Andrei Stecca, Silva, Roberto do Nascimento, Coelho, Alexandre Siqueira Guedes, Nagata, Tatsuya, Noronha, Eliane Ferreira, Ulhoa, Cirano José
Source:
Biological control 2012 v.61 no.2 pp. 134-140
ISSN:
1049-9644
Subject:
Fusarium solani, Hypocrea lixii, Phaseolus vulgaris, Trichoderma, beans, biological control, cDNA libraries, cell walls, chemical control, complementary DNA, expressed sequence tags, genes, irrigated farming, mycelium, plant pathogenic fungi, proteins, quantitative polymerase chain reaction, root diseases, root rot, transcriptomics, Brazil
Abstract:
The plant pathogen Fusarium solani causes a disease root rot of common bean (Phaseolus vulgaris) resulting in great losses of yield in irrigated areas of the Southeast and Midwest regions of Brazil. Species of the genus Trichoderma have been used in the biological control of this pathogen as an alternative to chemical control. To gain new insights into the biocontrol mechanism used by Trichoderma harzianum against the phytopathogenic fungus, Fusarium solani, we performed a transcriptome analysis using expressed sequence tags (ESTs) and quantitative real-time PCR (RT-qPCR) approaches. A cDNA library from T. harzianum mycelium (isolate ALL42) grown on cell walls of F. solani (CWFS) was constructed and analyzed. A total of 2927 high quality sequences were selected from 3845 and 37.7% were identified as unique genes. The Gene Ontology analysis revealed that the majority of the annotated genes are involved in metabolic processes (80.9%), followed by cellular process (73.7%). We tested twenty genes that encode proteins with potential role in biological control. RT-qPCR analysis showed that none of these genes were expressed when T. harzianum was challenged with itself. These genes showed different patterns of expression during in vitro interaction between T. harzianum and F. solani.
Agid:
147854