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Reciprocal Interference Between Specific CJD and Scrapie Agents in Neural Cell Cultures
- Nishida, Noriuki, Katamine, Shigeru, Manuelidis, Laura
- Science 2005 v.310 no.5747 pp. 493-496
- Creutzfeldt-Jakob Syndrome, coculture, immune system, mice, prions, scrapie, superinfection, virulence
- Infection of mice with an attenuated Creutzfeldt-Jakob disease agent (SY-CJD) interferes with superinfection by a more virulent human-derived CJD agent (FU-CJD) and does not require pathological prion protein (PrPres). Using a rapid coculture system, we found that a neural cell line free of immune system cells similarly supported substantial CJD agent interference without PrPres. In addition, SY-CJD prevented superinfection by sheep-derived Chandler (Ch) and 22L scrapie agents. However, only 22L and not Ch prevented FU-CJD infection, even though both scrapie strains provoked abundant PrPres. This relationship between particular strains of sheep- and human-derived agents is likely to affect their prevalence and epidemic spread.