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Blue-Light-Activated Histidine Kinases: Two-Component Sensors in Bacteria
- Swartz, Trevor E., Tseng, Tong-Seung, Frederickson, Marcus A., Paris, Gastón, Comerci, Diego J., Rajashekara, Gireesh, Kim, Jung-Gun, Mudgett, Mary Beth, Splitter, Gary A., Ugalde, Rodolfo A., Goldbaum, Fernando A., Briggs, Winslow R., Bogomolni, Roberto A.
- Science 2007 v.317 no.5841 pp. 1090-1093
- Brucella melitensis biovar Abortus, Erythrobacter litoralis, Pseudomonas syringae, algae, bacteria, blue light, chemotaxis, gene expression, histidine kinase, macrophages, mutants, photochemistry, photoreceptors, phototaxis, prokaryotic cells, receptors, virulence
- Histidine kinases, used for environmental sensing by bacterial two-component systems, are involved in regulation of bacterial gene expression, chemotaxis, phototaxis, and virulence. Flavin-containing domains function as light-sensory modules in plant and algal phototropins and in fungal blue-light receptors. We have discovered that the prokaryotes Brucella melitensis, Brucella abortus, Erythrobacter litoralis, and Pseudomonas syringae contain light-activated histidine kinases that bind a flavin chromophore and undergo photochemistry indicative of cysteinyl-flavin adduct formation. Infection of macrophages by B. abortus was stimulated by light in the wild type but was limited in photochemically inactive and null mutants, indicating that the flavin-containing histidine kinase functions as a photoreceptor regulating B. abortus virulence.