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Segregation of T-DNA copies in the progeny of a regenerant plant from a mannopine-positive hairy root line
- David, C., Tempe, J.
- Plant molecular biology 1987 v.9 no.6 pp. 585-592
- Daucus carota, Rhizobium rhizogenes, genetic transformation, DNA, plasmids, amino acid derivatives, roots, organogenesis, plant diseases and disorders, regeneration, phenotype, inheritance (genetics), opines
- We have analyzed opine content, T-DNA content, and developmental features of one hairy root culture line and its progeny. Opine-positive progeny still have the hairy, root phenotype and have essentially the same T-DNA structure as the parental plant. Opineless progeny do not exhibit the hairy root phenotype. Some of them do not contain any T-DNA sequences, whereas others contain only the left part of T-DNA. These results are compatible with the hypothesis that, in the hairy root line analyzed, the left part of T-DNA is integrated independently from the core T-DNA and can therefore segregate during sexual reproduction.