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Organisation and expression of a wound/ripening-related small multigene family from tomato
- Holdsworth, M.J., Schuch, W., Grierson, D.
- Plant molecular biology 1988 v.11 no.2 pp. 81-88
- Solanum lycopersicum var. lycopersicum, multigene family, plant proteins, messenger RNA, gene expression, restriction mapping, ethylene production, abiotic stress, ripening, leaves
- cDNA clones derived from a ripe tomato fruit cDNA library were used to investigate changes in the abundance of specific mRNAs in ripening fruit and wounded leaves. mRNAs related to one cDNA clone (pTOM 13) were expressed in both situations. This clone was used to identify homologous sequences in a tomato genomic library Three groups of related clones that hybridised to the pTOM 13 cDNA insert were identified and subcloned into plasmid vectors. Genomic Southern analysis of tomato DNA using gene-specific DNA fragments isolated from the subcloned DNAs indicated that all pTOM 13 closely related genes had been isolated. RNA dot blot analysis with these DNA fragments as probes indicated differential expression of this small multigene family in leaves and fruit.