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Activity of wall-bound enzymes in callus cultures of Gossypium hirsutum L. during growth

Kishor, P.B.K., Divakar Rao, J., Reddy, G.M.
Annals of botany 1992 v.69 no.2 pp. 145-149
Gossypium hirsutum, beta-glucanase, beta-fructofuranosidase, pH, cell wall components, enzyme activity, callus
Activities of alpha- and beta-glucosidase, alpha- and beta-galactosidase, alpha-mannosidase, beta 1,3-glucanase, acid and neutral invertases were detected in the cytoplasmic fraction as well as in cell walls isolated from callus cultures of cotton. Activity of beta-mannosidase, however, could not be detected in the cell walls. Transfer of callus to a fresh medium did not immediately influence the activities of alpha-glucosidase and beta-galactosidase but increased significantly beta-glucosidase, alpha-mannosidase, acid and neutral invertases. Addition of cycloheximide (1 and 100 mg l-1) further stimulated acid and neutral invertases but not other enzymes tested. Sodium chloride (NaCl) was effective in extracting alpha-glucosidase, beta-glucosidase, beta-galactosidase, acid and neutral invertases. EDTA extracted most of the alpha-galactosidase, alpha-mannosidase, beta-1,3-glucanase and some alpha-glucosidase. But, NaCl and EDTA could not extract some of the alpha- and beta-glucosidases and also acid and neutral invertases as evidenced from the residual and extra cellular activity. Studies with whole cells as a source of enzyme revealed that some of these enzymes were associated with the cell surface.