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Establishment of fast-growing callus and root cultures of Cephalotaxus harringtonia

Author:
Wickremesinhe, E.R., Arteca, R.N.
Source:
Plant cell reports 1993 v.12 no.2 pp. 80-83
ISSN:
0721-7714
Subject:
Cephalotaxus harringtonia, roots, cell culture, callus, alkaloids, biosynthesis, organogenesis, culture media
Abstract:
Callus cultures were established from Cephalotaxus harringtonia (Japanese plumyew) stem explants cultured on Murashige and Skoog medium supplemented with 4.5 micromolars 2,4-dichlorophenoxyacetic acid and 0.05 micromolars 6-furfurylaminopurine. The inclusion of 4.9 micromolars 6-(gamma,gamma-dimethylallylamino) purine as the sole hormone significantly increased the growth rate of the callus. Organogenesis giving rise to both shoots and roots occurred upon transfer of the callus onto a hormone-free medium. Vitrification was common on all regenerated shoots cultured on Gelrite-containing medium. Regenerated roots were excised and established in McCown's woody plant medium. Doubling the phosphate and nitrate levels in the medium increased the growth of these root cultures.
Agid:
1644959