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Acetoneâbutanolâethanol (ABE) fermentation using Clostridium acetobutylicum XY16 and in situ recovery by PDMS/ceramic composite membrane
- Wu, Hao, Chen, Xiao-Peng, Liu, Gong-Ping, Jiang, Min, Guo, Ting, Jin, Wan-Qin, Wei, Ping, Zhu, Da-Wei
- Bioprocess and biosystems engineering 2012 v.35 no.7 pp. 1057-1065
- Clostridium acetobutylicum, batch fermentation, butanol, ceramics, cleaning, culture media, permeates, pervaporation, solvents
- PDMS/ceramic composite membrane was directly integrated with acetoneâbutanolâethanol (ABE) fermentation using Clostridium acetobutylicum XY16 at 37Â Â°C and in situ removing ABE from fermentation broth. The membrane was integrated with batch fermentation, and approximately 46Â % solvent was extracted. The solvent in permeates was 118Â g/L, and solvent productivity was 0.303Â g/(L/h), which was approximately 33Â % higher compared with the batch fermentation without in situ recovery. The fed-batch fermentation with in situ recovery by pervaporation continued for more than 200Â h, 61Â % solvent was extracted, and the solvent in penetration was 96.2Â g/L. The total flux ranged from 0.338 to 0.847Â kg/(m2/h) and the separation factor of butanol ranged from 5.1 to 27.1 in this process. The membrane was fouled by the active fermentation broth, nevertheless the separation performances were partially recovered by offline membrane cleaning, and the solvent productivity was increased to 0.252Â g/(L/h), which was 19Â % higher compared with that in situ recovery process without membrane cleaning.