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Cyclophilin 20-3 relays a 12-oxo-phytodienoic acid signal during stress responsive regulation of cellular redox homeostasis

Park, Sang-Wook, Li, Wei, Viehhauser, Andrea, He, Bin, Kim, Soonok, Nilsson, Anders K., Andersson, Mats X., Kittle, Joshua D., Ambavaram, Madana M. R., Luan, Sheng, Esker, Alan R., Tholl, Dorothea, Cimini, Daniela, Ellerström, Mats, Coaker, Gitta, Mitchell, Thomas K., Pereira, Andy, Dietz, Karl-Josef, Lawrence, Christopher B.
Proceedings of the National Academy of Sciences of the United States of America 2013 v.110 no.23 pp. 9559-9564
acclimation, amino acids, binding proteins, biosynthesis, chloroplasts, cyclophilins, cysteine synthase, genes, homeostasis, jasmonic acid, metabolites, plant development, plant hormones, serine O-acetyltransferase, stress response, sulfur, thiols
The jasmonate family of phytohormones plays central roles in plant development and stress acclimation. However, the architecture of their signaling circuits remains largely unknown. Here we describe a jasmonate family binding protein, cyclophilin 20-3 (CYP20-3), which regulates stress-responsive cellular redox homeostasis. (+)-12-oxo-phytodienoic acid (OPDA) binding promotes CYP20-3 to form a complex with serine acetyltransferase 1, which triggers the formation of a hetero-oligomeric cysteine synthase complex with O -acetylserine(thiol)lyase B in chloroplasts. The cysteine synthase complex formation then activates sulfur assimilation that leads to increased levels of thiol metabolites and the buildup of cellular reduction potential. The enhanced redox capacity in turn coordinates the expression of a subset of OPDA-responsive genes. Thus, we conclude that CYP20-3 is a key effector protein that links OPDA signaling to amino acid biosynthesis and cellular redox homeostasis in stress responses.