Jump to Main Content
Evaluation of a lowâcost calorimetric approach for rapid detection of tuberculosis and other mycobacteria in culture
- RodrÃguez, D., Daniels, A.U., Urrusti, J.L., Wirz, D., Braissant, O.
- Journal of applied microbiology 2011 v.111 no.4 pp. 1016-1024
- calorimetry, developing countries, equipment, heat production, microbiology, monitoring, tetrazolium, tuberculosis
- Aims: The objective of this study was to evaluate the effectiveness of microcalorimetry in rapid detection of mycobacterium species using an inexpensive Isothermal microcalorimetry (IMC) instrument. In addition, we compared microcalorimetry with conventional monitoring techniques. Methods and Results: Isothermal microcalorimetry measures heat production rate and can provide rapid detection of living mycobacteria in clinical specimens. Using liquid medium showed that bacterial activity measured by IMC using a TAM AirÂ® agreed with the triphenyl tetrazolium chloride (TTC) assay. Using solid medium to enhance growth, fastâgrowing mycobacteria detection was achieved between 26 and 53âh and slowâgrowing mycobacteria detection was achieved between 54 and 298âh. In addition, the calorimetric data were analysed to estimate the growth rate and generation time of the mycobacteria monitored. Significance and Impact of the Study: Infections caused by mycobacteria are severe and difficult to treat. With 9Â·27 million new cases of tuberculosis in 2007, developing countries experience severe health and economic consequences owing to the lack of an affordable, fast detection method. Researchâgrade IMC instruments are too expensive to use in developing countries. Our study demonstrates that lessâexpensive instruments such as the TAM airÂ® are adequate for mycobacteria detection and therefore establishes a clear proof of concept.