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Temporally defined neocortical translation and polysome assembly are determined by the RNA-binding protein Hu antigen R

Author:
Kraushar, Matthew L., Thompson, Kevin, Wijeratne, H. R. Sagara, Viljetic, Barbara, Sakers, Kristina, Marson, Justin W., Kontoyiannis, Dimitris L., Buyske, Steven, Hart, Ronald P., Rasin, Mladen-Roko
Source:
Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.36 pp. E3815
ISSN:
0027-8424
Subject:
RNA-binding proteins, antigens, gene expression, genome, messenger RNA, neocortex, neurons, ribosomes, transcriptome, translation (genetics)
Abstract:
Significance The neocortex is an intricate and diverse cellular network in the brain, generating complex thought and voluntary motor behavior. Although recent attention has focused on the genome and transcriptome, our goal is to study the role of posttranscriptional processing and mRNA translation in neocortical development. In this work, we show that the protein components of actively translating ribosomes and their mRNA cargo in the developing neocortex depend on the temporally specific action of an RNA-binding protein, Hu antigen R (HuR). We further show that HuR is required for the development of neocortical neurons and structure. This study contributes to our overall understanding of how the regulation of functional gene expression influences neocortical development.
Agid:
1787137