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Replication initiator DnaA binds at the Caulobacter centromere and enables chromosome segregation

Author:
Mera, Paola E., Kalogeraki, Virginia S., Shapiro, Lucy
Source:
Proceedings of the National Academy of Sciences of the United States of America 2014 v.111 no.45 pp. 16100-16105
ISSN:
0027-8424
Subject:
Caulobacter crescentus, DNA replication, bacterial proteins, centromeres, chromosome segregation, chromosome translocation, mutation, provenance, replication origin
Abstract:
During cell division, multiple processes are highly coordinated to faithfully generate genetically equivalent daughter cells. In bacteria, the mechanisms that underlie the coordination of chromosome replication and segregation are poorly understood. Here, we report that the conserved replication initiator, DnaA, can mediate chromosome segregation independent of replication initiation. It does so by binding directly to the parS centromere region of the chromosome, and mutations that alter this interaction result in cells that display aberrant centromere translocation and cell division. We propose that DnaA serves to coordinate bacterial DNA replication with the onset of chromosome segregation.
Agid:
1787799