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Conditions for the attachment of Calystegia cells to solid substrates
- Kolster, A., Beiderbeck, R.
- Annals of botany 1987 v.59 no.4 pp. 471-473
- Calystegia sepium, mesophyll, cells, adhesion
- Mechanically isolated mesophyll cells of Calystegia sepium L. incubated in thin layers of non-agitated liquid media readily attach to their substrate (culture vessel). Conditions for this attachment of living plant cells are: a medium consisting of a low concentration of at least one macroelement and one sugar, a pH value between 5 and 7, and a substrate with a hydrophilic character (e.g. glass, polystyrene).