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Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

Fujita, Megumi, Kato, Hirotomo, Cáceres, Abraham G., Gomez, Eduardo A., Velez, Lenin, Mimori, Tatsuyuki, Zhang, Feifei, Iwata, Hiroyuki, Korenaga, Masataka, Sakurai, Tatsuya, Katakura, Ken, Hashiguchi, Yoshihisa
Acta tropica 2012 v.121 no.2 pp. 93-98
Lutzomyia, genes, genetic variation, genotyping, leishmaniasis, monitoring, restriction fragment length polymorphism, restriction mapping, ribosomal RNA, Andes region, Peru
Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru.