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Characterization of Technetium Species Induced in Spinach
- Harms, A. V., Krijger, G. C., van Elteren, J. T., de Goeij, J. J. M.
- Journal of environmental quality 1999 v.28 no.4 pp. 1188-1194
- 8-hydroxyquinoline, Spinacia oleracea, acetone, ammonium sulfate, biochemical pathways, chloroform, ethyl ether, gel chromatography, hydrophilicity, ion pair chromatography, leaves, nutrient solutions, polysaccharides, proteins, reversed-phase liquid chromatography, spinach, technetium
- Plants have the ability to accumulate the long-lived fission product Tc. In this work, an attempt was made to separate and characterize technetium species induced by spinach plants (L.) grown on a TcO containing nutrient solution. Combination of data obtained with selective extraction (ammonium sulfate, acetone, diethyl ether, and 8-hydroxyquinoline in chloroform) and chromatography (size-exclusion chromatography and reversed-phase ion-pair chromatography) gave us insight into Tc speciation in spinach plants. The following classes of Tc species in spinach leaf homogenate were found after an incubation period of 11 d: TcO (ca. 7%), Tc-cysteine (ca. 25%), Tc bound to insoluble cell-wall polysaccharides (ca. 17%), Tc bound to proteins (ca. 26%), and hydrophilic non-protein Tc species (ca. 25%). These results may yield a new insight into the metabolic pathways of Tc in plants.