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Phosphate inhibits acetotrophic methanogenesis on rice roots
- Conrad, R., Klose, M., Claus, P.
- Applied and environmental microbiology 2000 v.66 no.2 pp. 828-831
- acetates, carbon dioxide, hydrogen, isotope fractionation, methane, methane production, methanogens, phosphates, radiolabeling, rice, roots, stable isotopes
- The contribution of acetate- and H2/CO2-dependent methanogenesis to total CH4 production was determined in excised washed rice roots by radiolabeling, methyl fluoride inhibition, and stable carbon isotope fractionation. Addition of > or = 20 mM phosphate inhibited methanogenesis, which then was exclusively from H2/CO2. Otherwise, acetate contributed about 50 to 60% of the total methanogenesis, demonstrating that phosphate specifically inhibited acetotrophic methanogens on rice roots.